FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4d4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4d4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences460326
Sequences flagged as poor quality0
Sequence length151
%GC59

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGAG651.0903324E-555.7897532
GTACTAT400.006040021354.3950041
TAATCTC450.00962185948.351124
TATAAGC2050.045.992533
CTATAAG1900.045.806322
ATAATAC1002.7240058E-643.5160063
TGTTTAC907.479077E-540.2750938
CCTATAA2005.456968E-1239.8896751
TTCCGAT10150.039.300172
CTTCCGA10050.038.969561
TATACTG951.0258012E-438.1719365
TAAGCAA2350.037.03495
ATAAGCA2800.036.263344
CCTTTTA1604.5656634E-836.2633361
TAAGGGT800.002031802436.2633364
TCGTATA1051.8481647E-434.5365142
TAATACA1801.2856617E-732.234084
TTATAGG900.003619683732.234082
CTGTATG1402.744733E-531.0693579
TCCGATC12450.030.8748113
Produced by FastQC (version 0.11.2)