FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4d1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4d1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences608509
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC11170.18356343127217511No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA10930.17961936470947842No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA9340.15348992373161285No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG8950.1470808155672307No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT8940.1469164794604517No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC8820.14494444617910335No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT8220.13508427977236162No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC6960.114377930318204No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT6760.11109120818262343No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT6540.1074758138334848No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT6490.10665413329958966No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA7300.047.666781
CCGATCT8150.042.6953964
TTCCGAT8300.041.923792
CGATCTG5100.035.5358925
TCCCCCC3550.032.6738
TGCCTAC2507.094059E-1131.8970171
TCTTCCG5550.030.0422365
CGAGTCG5350.029.8102976
GTCGCGC4600.028.3669158
CTACACC5650.028.2274484
ATACTCC4900.028.1096174
ATACAGG1555.5004377E-528.0618926
CCATACT5700.027.979842
GTCTACG6500.027.882011
CATACTC5600.027.1849583
CCTACAC5100.027.0072783
CCCCCCG4300.026.9742229
TGTAGGA4850.026.90472
TATAGGT5200.026.4879073
TCCGATC13500.026.3123553