FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4d12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4d12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences560077
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA7130.12730392428183981No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC6960.12426862734945374No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA6030.1076637676605181No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT8150.044.483022
CTTCCGA8900.040.7344471
CCGATCT8950.040.5068864
GTATTAT2404.3655746E-1133.2325251
GTCTAGG1601.8736882E-631.7219541
CATACTC4500.030.6142023
CGATCTA2859.094947E-1230.5294025
GCTATAC950.004723204830.52943
ATACCCG3001.8189894E-1129.0029285
CGATCTG5600.028.4850185
TACTGCG1306.482299E-427.8824527
GTATATA3650.027.811031
TATAATA2207.469498E-726.3662972
CTCCCCC4950.026.361597
TCCCCCC5000.026.0979758
TCCGATC14300.025.8592533
ACCTATA3108.54925E-1025.7284031
CCATACT5100.025.5908182
TACTCCC5400.025.5118375
ACCATAC4900.025.1556021
Produced by FastQC (version 0.11.2)