FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4d11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4d11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences685657
Sequences flagged as poor quality0
Sequence length151
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATACATAGGTGTACGAAGCGAACGCGGCGAACTGAAACATCTAAGTAG12070.1760355396357071No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG11490.16757649962007243No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG8950.13053173817229316No Hit
ATCGTACACTGAATACATAGGTGTACGAAGCGAACGCGGCGAACTGAAAC8680.12659390919949773No Hit
GTACTGGTTCACTATCGGTCGATTACGAGTATTTAGCCTTGGAGGATGGT8390.12236438919168038No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC7450.10865491054565184No Hit
ATACATAGGTGTACGAAGCGAACGCGGCGAACTGAAACATCTAAGTAGCT7200.10500877260787829No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT22350.060.6731072
CTTCCGA22550.060.134991
CCGATCT23600.057.4552964
CGATCTA5550.045.7273035
TCCGATC33200.041.2785263
CGATCTG13500.033.83825
CGATCTC10650.032.680765
TGCTCTT12650.032.6750682
CTAGGGG2705.456968E-1232.226863
GATCTAG2351.2587407E-930.8555056
CGATCTT10450.029.8368265
GTGCTCT15000.029.4897271
TTATAGA1504.3924014E-529.0041734
TCTTCCG15600.027.4238175
TTATACT1606.8183734E-527.1914124
CATACTC3204.1836756E-1127.1914123
TAAGGTG5100.027.0136895
GCTATAT1358.0698763E-426.8576761
ATAAGGT3255.0931703E-1126.7730834
GATCTTT6350.026.263626
Produced by FastQC (version 0.11.2)