FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4d10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4d10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1221100
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA15870.12996478584882482No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC15770.12914585210056506No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC13010.10654328064859553No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA12830.10506919990172796No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT12260.1004012775366473No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT17500.041.840672
CCGATCT18800.038.9490284
CTTCCGA19350.038.5944561
CGATCTG9700.027.6543255
GTACAGG2654.5565685E-927.360421
CGATCTA5150.026.747275
CCTACAC6700.024.8877773
GTGTAGG6600.024.1683731
TCCGATC31900.023.4088273
TAGTATG7250.022.9987962
TCGCGCG7250.022.9969149
ATACCCG7300.022.8422075
TATAGGT7600.021.9405423
CCTATAA15100.021.6074871
TAGGTCG9200.021.2769365
GGTCGCG7850.021.239197
ACTACCG7200.021.1455965
GTCGCGC7550.021.1229938
GTCTAGA3457.50224E-821.0159761
CCGTGGG8350.020.835539
Produced by FastQC (version 0.11.2)