FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4c9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4c9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences263988
Sequences flagged as poor quality0
Sequence length151
%GC60

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAAGG259.377722E-487.003075
CCTAGGA450.00962999148.335042
TCTAGAC450.00962999148.335042
TATTGGA657.3254586E-444.6169552
TATACGG657.3254586E-444.6169552
TTCCGAT4650.040.5390622
CTTCCGA4950.039.539361
GTCCTAA800.002035444636.244411
ACCTATA1406.593036E-736.244411
TCCGATC5450.035.9186973
CTATACG850.002740417134.112391
CTATAAG1102.4308122E-432.9557082
GTGCTCT3600.032.2172551
TATAAGC1153.1551882E-431.522853
ATACCCG1652.3769935E-630.7586615
TGTAGGT950.00472024330.5273932
CCGATCT7000.030.0367744
TAGCCCG1453.4799275E-530.0010575
ATATTAT1453.484434E-529.9953731
TCTTCCG4150.029.6998425
Produced by FastQC (version 0.11.2)