FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4c8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4c8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences469679
Sequences flagged as poor quality0
Sequence length151
%GC57

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG15610.3323546507295408No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC9910.2109951690409833No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG9630.20503365064224716No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC7930.16883871750706334No Hit
TCTCCGGACTCCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAA7160.1524445419105389No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGC6780.14435390979796842No Hit
TTTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGATAACTCTTCCCGAGGC6570.13988277099891627No Hit
GTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGCGG6510.13860530277061567No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC5670.1207207475744072No Hit
ATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAGCTCG5220.11113973586215267No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT5030.10709441980586741No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA5020.10688150843448398No Hit
ACCTCCGGCTGAGCCGTTTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGA4980.10602986294895025No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGGG702.3298162E-762.1506652
TTCCGAT15750.053.4035342
CTTCCGA16000.052.57471
TCCGATC18550.045.733513
CCGATCT18950.044.385524
ACGTACT907.470837E-540.2828375
ATATAGG951.0266538E-438.166751
ATAATGG951.027298E-438.1626853
TAAAGGT1001.3892986E-436.254554
CTATACA1051.850856E-434.5281454
ATCTAGG850.002738823934.1219332
CATACTC3000.033.837583
CCATACT3100.032.7460482
CGATCTA2900.032.504085
TAATAGT1352.1379603E-532.226274
GATCTAG1652.3785597E-630.7647131
CGATCTG11250.030.2926965
GTGCTCT9650.030.058791
TACAGGA1952.5994632E-729.7473242
TGCTCTT9050.029.6446062
Produced by FastQC (version 0.11.2)