FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4c7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4c7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1091098
Sequences flagged as poor quality0
Sequence length151
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC54810.5023380118009565No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG53830.4933562338121782No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA42890.39309026320275536No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA40070.36724473878606684No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG39470.36174569103783527No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT38930.35679654806442684No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT37050.33956619845330116No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC35610.3263684838575453No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCC31390.28769184802831643No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTC30850.282742705054908No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT28760.26358768873190125No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT27640.25332279960186893No Hit
CTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTT26910.2466322915081872No Hit
CTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTCTTAATCG26040.23865867227325135No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA25920.23755886272360502No Hit
TCTGAGAAGGGTTCGAGTGTGAGCATGCCTGTCGGGACCCGAAAGATGGT25660.23517594203270467No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG25280.231693211792158No Hit
GGTAGGACGTGTCGGCTGCTTTGTTGAGCCGTCACACGGAATCGAGAGCT25050.22958524348866924No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT24920.228393783143219No Hit
GTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCT24840.22766057677678814No Hit
ATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAGCTCG24740.22674406881874956No Hit
GTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGC24190.22170327504953727No Hit
AAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGA24110.22097006868310637No Hit
TTGTTACACACTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCT23560.21592927491389408No Hit
GTCTTAATCGACCAACACCCTTTGTGGGTTCTAGGTTAGCGCGCAGTTGG23230.21290479865236667No Hit
GATTAGAGGCATTGGGGGCGCAACGCCCTCGACCTATTCTCAAACTTTAA23090.21162168751111265No Hit
GTGCCCAACTGCGCGCTAACCTAGAACCCACAAAGGGTGTTGGTCGATTA22960.21043022716566248No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC22510.2063059413544888No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG22300.20438127464260772No Hit
TTCCCAAACAACCCGACTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCC22030.2019067031559035No Hit
CTCCTACTCATCGAGGCCTGGCTCTTGCCCCGACGGCCGGGTATAGGTCG21360.19576609983704488No Hit
TATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAA21020.19264997277971366No Hit
CTACTAGATGGTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAA20900.19155016323006732No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAAT20890.19145851243426346No Hit
GTTACACACTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGT20780.19045035368042101No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT20690.18962549651818625No Hit
TCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGA20350.18650936946085503No Hit
GGGTAAAGCCAGAGGAAACTCTGGTGGAAGCCCGCAGCGATACTGACGTG19720.18073536932521186No Hit
CCCTTAGTAACGGCGAGCGAACCGGGAAGAGCCCAGCTTGAAAATCGGAC19350.17734428988046902No Hit
TAGTAACGGCGAGCGAACCGGGAAGAGCCCAGCTTGAAAATCGGACGTCT19080.1748697183937648No Hit
CTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTACTT18810.1723951469070606No Hit
GTACTTGTTCGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATT18770.17202854372384516No Hit
CTATTGTGTTGGCCTTCGGGATCGGAGTAATGATTAACAGGGACAGTCGG18530.1698289246245525No Hit
CATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGAGTAATG18360.1682708610958869No Hit
TCTCCGGACTCCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAA18210.166896099158829No Hit
TTTCAAGACGGGTCGAATGGGGAGCCCACAGGCCGACGCCCGGAGCACGC17720.16240521016443987No Hit
GTCAAAGAGTGCTTGAAATTGTCGGGAGGGAAGCGGATGGGGGCCGGCGA17690.16213025777702827No Hit
GTCTTGAAACACGGACCAAGGAGTCTGACATGTGTGCGAGTCAACGGGTG17050.15626460684558124No Hit
CCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTGCGGGCT16850.15443159092950404No Hit
GATTAAGACAGCAGGACGGTGGTCATGGAAGTCGAAATCCGCTAAGGAGT16750.15351508297146546No Hit
AGGTAGGACGTGTCGGCTGCTTTGTTGAGCCGTCACACGGAATCGAGAGC16740.1534234321756616No Hit
GTCCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGA16620.15232362262601526No Hit
CCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTCTTAATCGAC16620.15232362262601526No Hit
GATCTGCACCGACGGCCGCTCCGCCCGGGCTCGCGCCCTAGGTTTTGCAG16150.14801603522323384No Hit
GGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCC15690.14380009861625628No Hit
CCCATGTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCGGCCTTCA15670.14361679702464858No Hit
ACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAGCG15630.14325019384143314No Hit
CCTCTAATCATTGGCTTTACCCGATAGAACTCGCGTCCGAGCTCCAGCTA15480.14187543190437524No Hit
GTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGCGG15340.14059232076312117No Hit
GTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCG15060.13802609848061312No Hit
AACCTAGAACCCACAAAGGGTGTTGGTCGATTAAGACAGCAGGACGGTGG14830.13591813017712434No Hit
TCCATGACCACCGTCCTGCTGTCTTAATCGACCAACACCCTTTGTGGGTT14730.1350016222190857No Hit
GGCTTTACCCCGCTCAGGCATAGTTCACCATCTTTCGGGTCCCGACAGGC14560.13344355869042013No Hit
CTCCACAAGCGTATCAAAAGCCCGGGCTTAGGCCGCCACCGTAATCCGCG14510.1329853047114008No Hit
GCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGATAACATCATA14460.1325270507323815No Hit
ATTTAAAGTTTGAGAATAGGTCGAGGGCGTTGCGCCCCCAATGCCTCTAA14440.1323437491407738No Hit
GTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCG14420.13216044754916606No Hit
TTCTAGGTTAGCGCGCAGTTGGGCACCGTAACCCGGCTTCCGGTTCATCC14140.12959422526665798No Hit
GGCCTAAGTTCCCTGGAAAGGGGCGCCAGAGAGGGTGAGAGCCCCGTCGT13820.12666139980093447No Hit
ATACTGACGTGCAAATCGTTCGTCTGACTTGGGTATAGGGGCGAAAGACT13780.12629479661771903No Hit
ACCTAGAACCCACAAAGGGTGTTGGTCGATTAAGACAGCAGGACGGTGGT13600.12464508229324954No Hit
ATCCTATTGTGTTGGCCTTCGGGATCGGAGTAATGATTAACAGGGACAGT13310.12198720921493761No Hit
GTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTCCGTCCAAGGCTAAAT13310.12198720921493761No Hit
TCTTTACCTCGCGGTACTTGTTCGCTATCGGTCTCTCGCCCGTATTTAGC13260.12152895523591832No Hit
GGCTTAGGCCGCCACCGTAATCCGCGTCGGTCCACGCCCCGAATCGATCG13150.12052079648207585No Hit
GCGCTTAAGCGCGCGACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCT12920.11841282817858707No Hit
CTATCAGACGGGTTTCCCCCGACTCTTAGGATCGACTAACCCATGTGCAA12780.11712971703733305No Hit
TCCCTGGTCGGCATCGTTTATGGTTGAGACTAGGACGGTATCTGATCGTC12550.11502174873384427No Hit
CCCTCCTACTCATCGAGGCCTGGCTCTTGCCCCGACGGCCGGGTATAGGT12500.11456349475482494No Hit
GAATCGAGAGCTCCAAGTGGGCCATTTTTGGTAAGCAGAACTGGCGATGC12460.1141968915716095No Hit
GTCCTACCTATTTAAAGTTTGAGAATAGGTCGAGGGCGTTGCGCCCCCAA12100.11089746292267057No Hit
TTTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGATAACTCTTCCCGAGGC12100.11089746292267057No Hit
CATGTCAGACTCCTTGGTCCGTGTTTCAAGACGGGTCGAATGGGGAGCCC12080.11071416133106285No Hit
CTCTTAGGATCGACTAACCCATGTGCAAGTGCCGTTCACATGGAACCTTT12070.11062251053525897No Hit
AACAAGTACCGCGAGGTAAAGATGAAAAGGACTTTGAAAAGAGAGTCAAA12040.11034755814784739No Hit
CCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAGCGG11900.10906444700659336No Hit
CCCTTTGTGGGTTCTAGGTTAGCGCGCAGTTGGGCACCGTAACCCGGCTT11770.10787298666114317No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGC11590.1062232723366737No Hit
CCTAGAACCCACAAAGGGTGTTGGTCGATTAAGACAGCAGGACGGTGGTC11580.10613162154086983No Hit
CTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATTTACCGCCCGAT11520.10558171676604668No Hit
TAGGTAGGACGTGTCGGCTGCTTTGTTGAGCCGTCACACGGAATCGAGAG11500.10539841517443896No Hit
ATGCCTGAGCGGGGTAAAGCCAGAGGAAACTCTGGTGGAAGCCCGCAGCG11480.10521511358283124No Hit
CGTCCTACCTATTTAAAGTTTGAGAATAGGTCGAGGGCGTTGCGCCCCCA11480.10521511358283124No Hit
GTCTGGAGAAGCGTCCTCAGCGACGGACCGGGCCTAAGTTCCCTGGAAAG11410.1045735580122042No Hit
GTCCTAAGATGAGCTCAACGAGAACAGAAATCTCGTGTGGAACAAAAGGG11340.1039320024415772No Hit
GTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAA11320.10374870084996948No Hit
TGTCAGACTCCTTGGTCCGTGTTTCAAGACGGGTCGAATGGGGAGCCCAC11020.10099917697585369No Hit
GGATAGCTGGAGCTCGGACGCGAGTTCTATCGGGTAAAGCCAATGATTAG11000.10081587538424597No Hit
GTGTTGGCCTTCGGGATCGGAGTAATGATTAACAGGGACAGTCGGGGGCA11000.10081587538424597No Hit
GTTCTAGGTTAGCGCGCAGTTGGGCACCGTAACCCGGCTTCCGGTTCATC10920.10008266901781508No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA19300.087.163671
TTCCGAT19600.086.569452
CCGATCT22450.074.61064
CGATCTG12350.066.933385
TCCGATC30500.055.156033
TGCTCTT7700.052.735392
TCTTCCG8200.050.4041025
GATCTGA4750.048.8518646
CGATCTA4250.046.0659145
CGATCTT8100.044.759985
GGCGACC12350.044.6120268
GGGCGAC12500.044.0766837
CGATCTC7150.043.608115
ATCTGAG1053.826419E-641.4254537
GCGACCC13250.041.034659
TCTGTGC1451.929402E-839.996998
CTAGGGG3100.039.7641833
GTGCTCA15300.038.8532839
GTGCTCT11700.038.4247171
ATCTGAC3600.038.261017
Produced by FastQC (version 0.11.2)