FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4c6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4c6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences375120
Sequences flagged as poor quality0
Sequence length151
%GC56

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG8090.2156643207506931No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC4910.13089144806995096No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG4900.13062486670931966No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC4480.11942844956280656No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCA553.2141412E-452.7347874
GTATACG701.6911712E-551.8000031
CTTCCGA11550.044.57941
TATAACA1505.347829E-1043.5062034
TTCCGAT11850.042.838822
GTACAGT700.001054363141.4400021
CCGATCT13050.038.3387954
TCCGATC13700.037.5783463
GTATTAA1209.529873E-636.2600021
ATCTAGC1001.3918987E-436.240677
ATATAAC1051.8499627E-434.528733
CTATGGG850.002736106534.127062
TCTAAGG850.002736106534.127062
GATCTAG1501.1301236E-633.8381586
CGATCTA2705.456968E-1232.2268145
TTAGTGA1153.1557205E-431.5262343
TAACAGT1204.0498748E-430.2126396
CCATAAG1000.00606150529.0080011
GTGTAAT1000.00606150529.0080011
TGTAGAC1000.00606150529.0080012
Produced by FastQC (version 0.11.2)