FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4c5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4c5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences434488
Sequences flagged as poor quality0
Sequence length151
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG7320.16847415808952146No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG4450.10241939938502329No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGAC553.2109E-452.747453
CTTCCGA12450.049.5169371
TTCCGAT13750.045.8902852
GATCTAG1053.8090784E-641.4444246
CCGATCT16350.039.0363464
TCCGATC16650.038.7685853
GTATAGT951.02561666E-438.17251
CAATACT800.00203152936.2638784
ATCTAGG1051.8528425E-434.5211227
ATAGCAC900.003619199632.2345543
ATATGAC900.003619199632.2345543
ATAGGTG2309.949872E-1031.5338025
TATAAGC2558.731149E-1131.286483
ATATAGC950.004715547430.5379986
TAGGATG950.004715547430.5379985
TACTCCC2252.798879E-829.01115
CGATCTG8250.029.0110975
TGCTCTT9300.028.0752582
GTTCTAA1306.462521E-427.895291
TTAGAGC1050.007689357727.6296164
Produced by FastQC (version 0.11.2)