FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4c4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4c4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1171700
Sequences flagged as poor quality0
Sequence length151
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA39280.3352393957497653No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC38590.329350516343774No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA31310.26721857130664844No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT27800.23726209780660582No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC27710.23649398310147648No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG26700.2278740291883588No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT24920.2126824272424682No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT23480.20039259196039944No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT21320.18195783903729623No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA21060.17973884100025603No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC20640.17615430570965265No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT20580.17564222923956643No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT19450.16599812238627634No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT17280.14747802338482546No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA16800.14338141162413587No Hit
AAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGA16560.14133310574379107No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA16460.14047964496031407No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG16350.13954083809848938No Hit
GTCCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGA15670.13373730477084578No Hit
CTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGACCCGGCCAATTAA15140.1292139626184177No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG14530.12400785183920798No Hit
TATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGAACCTTGGGATGGGT14360.1225569685072971No Hit
ATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTC14260.12170350772382009No Hit
CTATTGTGTTGGCCTTCGGGATCGGAGTAATGATTAACAGGGACAGTCGG13970.11922847145173679No Hit
GTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCG13920.1188017410599983No Hit
ACACAATAGGATCGAAATCCTATGATGTTATCCCATGCTAATGTATCCAG13870.1183750106682598No Hit
CATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGAGTAATG13680.11675343517965349No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT13270.1132542459673978No Hit
TCCCTGGTCGGCATCGTTTATGGTTGAGACTAGGACGGTATCTGATCGTC12440.1061705214645387No Hit
GCCCCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACA12260.10463429205428011No Hit
TAGTTGAACCTTGGGATGGGTCGGCCGGTCCGCCTTTGGTGTGCATTGGT12070.10301271656567382No Hit
GTACAATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGC11920.10173252539045831No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCC11910.1016471793121106No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA12450.053.0031551
TTCCGAT13000.050.20292
CCGATCT13300.049.07474
TCCCCCC15050.048.1705938
CATACTC15450.047.878273
ATACTCC15700.047.5777974
CCATACT15350.047.2412382
ACCATAC14650.045.0436361
CTCCCCC17000.042.218697
CCCCCCG17350.041.3670169
CGATCTG7700.040.499025
ACTCCCC19150.037.870236
TACTCCC21600.034.5820125
GGGCGAC5950.032.897687
CGAGTCG15200.031.9667116
TACGAGT15700.031.8725054
GTCTACG16450.031.739221
TCGCGCG11600.031.2485929
GTCGGGT16350.030.1515469
ACGAGTC16400.030.0698875
Produced by FastQC (version 0.11.2)