FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4c3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4c3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences633084
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC14530.22951140764890599No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT10270.16222175888191773No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA10150.1603262758180589No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA9770.15432391278250596No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC9760.15416595586051773No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT8680.1371066082857883No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG8210.12968263295234123No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT7400.11688812227129416No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA7070.11167554384568242No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC6400.10109243007247062No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT9950.048.0915264
CTTCCGA10200.047.6198541
TTCCGAT11200.042.7207952
CCTACAC4100.042.4399723
CGATCTG5750.041.6096235
GGGCGAC3350.038.9560937
CTACACC4850.038.8668444
TCCCCCC5750.037.826938
GTCTAGG1553.468631E-837.4172361
TCGCGCG3950.034.8741959
CCGTGGG2103.783498E-1034.524589
TCTTCCG7900.033.9564555
ACTACCG1956.9812813E-933.4622885
TCCTACA5250.033.140982
GTCGCGC3800.032.4349338
CCATACT6950.032.3362962
GGTCGCG4050.032.2229427
TACGAGT5400.032.2229424
GGCGACC4100.031.8299798
GCGACCC4150.031.4464879