FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4c2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4c2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences511258
Sequences flagged as poor quality0
Sequence length151
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACACT855.32599E-542.663094
TCATAAC1053.8105827E-641.444143
TAGACGT1953.6379788E-1240.9128074
GTAGACT907.46075E-540.292924
TGTAGAC2002.237357E-1036.2565352
CTTCCGA16300.035.5927161
ACACATA2650.035.572445145
TTCCGAT16700.033.4341662
TACACTG1801.2860437E-732.2343375
TATAAGC2951.4551915E-1129.5026113
CCGATCT20250.029.01094
ATACTGC1255.139041E-429.0108996
TCCGATC20100.028.1449033
TCATACT1050.007690863727.6294294
TAAGCAA3204.1836756E-1127.1977215
CCTATAA3204.1836756E-1127.195061
TTATACT1358.059562E-426.8619444
GTTTAGA1907.1310624E-626.7179531
ATAAGCA3357.4578566E-1125.9799124
GTATATA1450.001223638225.0069481
Produced by FastQC (version 0.11.2)