FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4c12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4c12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences816990
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC19450.2380690094125999No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA18100.22154493935054284No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA15370.1881295976694941No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT14490.1773583519994125No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC14220.17405353798700107No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG13380.1637718943928322No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT12800.15667266429209661No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT11120.13610937710375892No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT11090.13574217554682433No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC11010.1347629713949987No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT10970.1342733693190859No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA9960.12191091690228767No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT9580.11725969718111605No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTG9060.11089487019424964No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT9060.11089487019424964No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA8400.10281643594168839No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCCCC6800.042.641488
CCATACT7700.038.6201172
CCCCCCG7600.038.15299
CCGATCT8250.036.92464
ACCATAC7350.036.5119321
TTCCGAT8350.036.482392
GTACGGG1001.3877917E-436.2652321
CTCCCCC8450.034.3150377
CATACTC9400.033.9504283
CTTCCGA9600.031.7320771
ACTCCCC9150.031.7073086
ATACTCC9450.031.4682454
GTAGTAT3250.031.2438931
GTATACA3052.3646862E-1128.5365771
TACTCCC11450.027.2385165
CGATCTG6650.026.1764095
GTCTACG9450.026.0956171
TGCCTAC3900.026.0365751
CGAGTCG9050.025.6461336
CCTACAT3751.0913936E-1125.1438963
Produced by FastQC (version 0.11.2)