FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4c11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4c11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences460771
Sequences flagged as poor quality0
Sequence length151
%GC56

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG1109.930045E-846.144411
CTTCCGA13250.043.2339551
TTCCGAT13500.041.8961942
TCCGATC14750.038.8414963
TACAGTA750.001482866138.6650167
TATTAGA850.002738114434.12362
CTCTATA850.002738114434.12362
CCGATCT16700.033.8718574
TTATACC1301.6496537E-533.47101145
TCTAACT900.00362697432.2208488
ATCTGAG1352.1403344E-532.2208487
TACACTG1601.8700757E-631.7277265
TCCTATG1601.8716601E-631.7242832
CTATAAT1204.0505416E-430.2136021
ATACAGT1453.4796212E-530.0084926
TAGACGT1703.000443E-629.8613914
ATCTAAC1000.006072369428.9987627
ACTGTGT1000.006072369428.9987628
TTACATG1306.466891E-427.8925083
ATACACT1306.466891E-427.8925084
Produced by FastQC (version 0.11.2)