FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4b9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4b9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412514
Sequences flagged as poor quality0
Sequence length151
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG11430.27708150511255375No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC8890.21550783730976403No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA8240.19975079633660917No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT7520.18229684325865303No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC7390.17914543506402206No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG7180.1740546987496182No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA6750.16363081010583883No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA6570.1592673218363498No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC5900.14302544883325172No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT5780.14011645665359235No Hit
TCTCCGGACTCCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAA5390.13066223206969946No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG5150.12484424771038075No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT5110.12387458365049428No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGC5110.12387458365049428No Hit
TTTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGATAACTCTTCCCGAGGC5070.12290491959060783No Hit
GTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGCGG5040.12217767154569299No Hit
AAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGA4850.11757176726123235No Hit
CTATTGTGTTGGCCTTCGGGATCGGAGTAATGATTAACAGGGACAGTCGG4360.10569338252762331No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT4290.10399647042282202No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTGTA554.0578416E-665.907837
CTCTAGG400.00604013654.3937381
AGACTCT604.9303396E-448.3558546
TTCCGAT16950.048.3499872
CTTCCGA17000.048.2077831
CCGATCT18450.045.2052734
TCCGATC20900.039.5590823
ATCTAGC951.02795675E-438.157167
ATATACT1001.3872176E-436.262494
GATCTAG1655.977563E-835.1678926
TATACTC900.003617515832.2372365
CGATCTT7050.031.8942875
ATACAGT950.00471335630.5405396
ATAATAC950.004716153330.5368351
ATCTTAC950.00472455430.525737
TATCGTG950.00472455430.525737
TATAGGT4700.029.318613
TAATACA1504.382208E-529.0099934
CCCTAAT1255.1382795E-429.0099931
ATAGAGT1000.006060165429.009994
Produced by FastQC (version 0.11.2)