FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4b8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4b8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1109591
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC25190.22702058686488985No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA23750.21404283199845708No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA19870.17907499249723546No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC19860.1789848691995519No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG19370.17456882761305742No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT19110.17222562187328483No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT17640.15897749711380138No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT14810.13347260386935367No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT14640.13194050780873315No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC14570.1313096447249482No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT13430.12103558878902226No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT12870.11598868411874284No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT12670.11418621816507164No Hit
ATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA12460.11229362891371686No Hit
TAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTA12180.10977017657857716No Hit
GAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGA11650.10499364180134843No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA16050.060.532891
TTCCGAT16500.058.0031552
CCGATCT17450.055.2658654
CGATCTG10250.039.6154825
GGGCGAC5400.037.5912487
TCCCCCC9300.037.418028
CGATCTA5800.036.255245
CCATACT11600.035.0019042
CCCCCCG10350.032.9215289
TCGCGCG8050.032.4212049
GTCGCGC7900.032.1191068
ACCATAC12050.031.88971
ATACTCC12350.030.5307274
TATAGGT8800.030.487363
TCTTCCG10550.030.2413375
CTCCCCC12000.030.2072537
TCCGATC32350.030.0352543
CATACTC12850.029.9070473
GGTCGCG9200.028.3685517
ACTCCCC13300.027.8047716