FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4b7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4b7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1054003
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC17850.16935435667640417No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA16340.1550280217418736No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG14440.1370015075858418No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC14170.13443984504787937No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA13460.12770362133694116No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT13450.12760874494664626No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT12900.12239054348042652No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC12370.1173620947947966No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT10610.10066385010289346No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT13500.045.112294
TTCCGAT15550.039.631262
CTTCCGA15250.038.9846271
GTCGCGC7800.037.1751678
CGATCTG7600.036.250955
TCGCGCG7800.036.245799
TCCCCCC8450.035.1734288
TAGGACA4200.034.524714
GGTCGCG8700.033.329467
CTCCCCC9200.032.306037
TATAGGT9000.032.2230643
CCCCCCG9600.030.2048269
AGGTCGC9550.029.6081076
CATACTC11150.029.2608553
GGGCGAC5250.028.9966327
CCATACT10300.028.8599782
TCTAGGG2553.0231604E-928.432122
GTACAGG2853.237801E-1027.983191
ACCATAC10150.027.8578741
CTAGGGG1606.827264E-527.1882113