FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4b6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4b6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences280740
Sequences flagged as poor quality0
Sequence length151
%GC52

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTATA350.003562391562.1491972
ATAGTGT855.328432E-542.651416
TTCCGAT11900.038.9955752
CTTCCGA11950.038.8324131
CCGATCT12850.036.676894
TCCGATC13300.035.9811133
ATACAGT1102.4302531E-432.957916
GTGTTAA1601.8692717E-631.7219851
TAGACGT1153.154463E-431.5249544
ACTGTAC950.00471921130.5294273
TTAGAGT1204.0482657E-430.2114144
ATTACAC1204.0482657E-430.2114143
TTATACT1204.0482657E-430.2114144
GTATATA1204.0482657E-430.2114141
CAAGACA1453.479126E-530.0030574
TAGAGTG1255.1414437E-429.0029565
CTACTCA1306.467388E-427.887464
TGGGTCG1306.467388E-427.887465
TATACTG1306.467388E-427.887465
TTAATGC1050.00769533127.6218643
Produced by FastQC (version 0.11.2)