FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4b4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4b4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1060691
Sequences flagged as poor quality0
Sequence length151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC16990.16017860055378993No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA15440.14556548514128997No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG13640.1285954156299997No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT12400.11690492329999973No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA12200.11501936002096746No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC12090.11398230021749972No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT10790.10172613890379008No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA12400.044.437751
TTCCGAT13150.042.454642
CCGATCT13150.041.3558244
TCCCCCC6800.040.5148668
CCCCCCG7600.037.2040949
CGATCTG8300.034.944845
CTCCCCC8800.031.306947
ACTCCCC9750.029.0042156
CCATACT10650.028.5930082
ACCATAC10300.028.1567781
TCGCGCG5000.027.5501069
GTCGCGC4900.026.6327558
GGTCGCG5300.025.9906677
ATACTCC11200.025.8966224
GTATAAG4850.025.4136681
TATAGGT5800.025.0036333
TCCGATC22350.024.3323963
TACTCCC12050.024.069895
CATACTC13100.023.8011683
AGGTCGC5850.023.5504326
Produced by FastQC (version 0.11.2)