FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4b3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4b3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences577003
Sequences flagged as poor quality0
Sequence length151
%GC51

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG7560.13102184910650377No Hit
ATTATGGACCGTACCCGAAACCGACACAGGTGGACTGGTTGAGTATACCG6660.1154240099271581No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA20950.054.681331
TTCCGAT21150.053.478632
CCGATCT20950.052.9555054
TCCGATC22400.050.1753
GACCGTA4700.047.8222357
ACCGTAC4900.047.3499958
TATGGAC4700.046.2835923
CGATCTG11350.042.1649675
CGATCTA3550.040.851255
ATTATGG5800.037.502421
TTATGGA6000.037.460752
GTGCTCT14600.034.265911
TCTAGAC850.002738996934.1228073
ACACATA4250.034.11985145
ATGGACC6250.033.645094
TGCTCTT14100.033.4241452
GATCTAG2051.1361408E-831.8313261
TGGACCG7850.031.4060235
TCTTCCG14950.030.5564615
GTCTATA1204.054406E-430.2102831
Produced by FastQC (version 0.11.2)