FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4b2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4b2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences449799
Sequences flagged as poor quality0
Sequence length151
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG5320.11827505174533515No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC5190.11538487190945287No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA8700.036.6681821
CCTAATA800.00203501236.25152
ATAGAGT1051.8516478E-434.5252383
TATACGT850.002739831834.119064
TTCCGAT9250.033.7040982
TATACCA1153.1585852E-431.5230465
TATACTT1000.006069720729.0012045
TAATACT1606.817997E-527.1886254
TATACTA1606.817997E-527.1886255
TATATCG1100.00966408426.3647275
CTCATAC1100.00966408426.3647273
CGTATAC1409.983603E-425.8939293
CCGATCT12350.025.831034
CTAGACG1450.001225097725.0010363
TAAGTGG1450.001225097725.0010365
TCCGATC12500.024.9410343
CTAGGGG1500.001492636924.167674
GTTATGA1500.001492636924.167671
ATACTCC2101.5520996E-524.1676654
TGCTCTT7600.023.8496722
Produced by FastQC (version 0.11.2)