FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4b1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4b1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences755676
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC14650.19386615427775925No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG11730.1552252552681308No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA11400.15085830435265907No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA11380.1505936406608123No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT10450.1382867789899375No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC9730.12875888608345376No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT9170.12134830271174418No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT8370.11076175503787336No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC8160.10798278627348228No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT7590.10043987105584933No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT7050.041.140954
TTCCGAT8100.035.8031232
CTTCCGA7900.035.7965281
CCCCCCG7700.032.9507879
GTCGCGC5150.032.3749478
TCCCCCC7900.032.1165858
TCGCGCG5200.032.063659
CCATACT8650.031.8502942
ACCATAC8900.030.1449891
TATAGGT6000.029.004373
ATACTCC10100.028.7171974
CTCCCCC8850.028.6690467
CATACTC10400.028.5860373
TAGACCG2553.0158844E-928.4356565
GTACTAT2054.0316263E-728.2969471
GGTCGCG5900.028.2594877
ACTCCCC9800.028.116486
GTGTAGG4850.026.9112681
CCTACAC5150.026.7516043
TATATAC4150.026.2087673
Produced by FastQC (version 0.11.2)