FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4b12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4b12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences565533
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC10350.18301319286407688No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA8980.1587882581564648No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG8440.1492397437461651No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA7700.13615474251723597No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT7410.13102683663022316No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT7340.12978906624370284No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC7280.128728120198114No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA8500.051.178841
TTCCGAT8900.048.878672
CCGATCT9100.047.8044174
GTCCTAA3900.037.181211
CGATCTG5200.034.8573845
CCTACAC3850.032.0144733
TCGCGCG3450.029.4190529
GGGTACC1504.3913293E-529.003916
TATAGGT4000.029.0013453
TGTAGGG1255.149646E-429.0013432
TCCGATC15150.028.7142053
GGGCGAC2603.7143764E-927.8834427
CGATCTA2903.947207E-1027.5012745
GTCGCGC3700.027.431288
GGTCGCG3850.026.3625287
GTCTAGA2901.1878001E-825.0011581
CAGGGGC1450.001225452825.0011584
AGTTAGG1450.001225452825.0011582
ATAGGGT1500.001493068624.1677883
TCCTACA4800.024.1677882
Produced by FastQC (version 0.11.2)