FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4b11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4b11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences751919
Sequences flagged as poor quality0
Sequence length151
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTACTTGTGCGCTATCGGTCTCTGGCCAATATTTAGCTTTAGATGAAA15110.20095249621302297No Hit
GTTTATGGTTAAGACTACGACGGTATCTGATCGTCTTCGATCCCCTAACT10860.14443045062034607No Hit
ACCATACTCCCCCCCGAGCCCAAAAACTTTGATTTCTCGTAAGGTGCCGA9800.13033318748429018No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACTT5700.080.1535642
TTCCGAT32450.079.336082
CTCTACT5950.078.0045851
CTTCCGA33050.077.895791
CTACTTG6350.071.948873
CCGATCT36100.071.917244
ACTTGTG7600.058.2067575
CGATCTA7100.055.155975
TACTTGT8650.053.6563344
TGTGCGC8800.053.5480168
TCCGATC50000.051.4891243
CGATCTG20400.049.4130635
CGATCTT11750.047.523675
TTGTGCG9800.047.344187
ATCTGAG1850.047.024297
GTATATA1505.347829E-1043.5119361
CCATACT10600.042.4172972
CGATCTC15500.042.1083265
TGCTCTT13850.041.3651352
GTGCGCT11300.041.0595559
Produced by FastQC (version 0.11.2)