FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4b10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4b10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences426738
Sequences flagged as poor quality0
Sequence length151
%GC58

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGGA604.943844E-448.3294263
TATAGTG657.3347555E-444.611785
CTATACT855.3388707E-542.6436124
CTTCCGA17100.042.3992041
TTCCGAT17300.041.909042
TCCGATC17350.041.7833633
TAGTACT750.001483021138.663544
CCGATCT19900.036.4292144
AGGGTAG1406.6019675E-736.247075
TAAACCT850.002741318234.1148874
GGTTATG1102.4333003E-432.9557461
AATAGAG950.004723448330.5274282
CCTATAA1902.0711923E-730.5274281
ACTATAC950.004726157530.523853
CTATGCT1504.3952925E-528.9976564
TATAGAC1000.00607300628.9976543
TACTAAG1000.00607300628.9976545
AACGTAT1306.475433E-427.885631
GGCCCTA1050.007711028727.6135777
GTATTGT1100.00966377626.3645951
Produced by FastQC (version 0.11.2)