FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4a9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4a9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences447338
Sequences flagged as poor quality0
Sequence length151
%GC57

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGAG300.001935869672.492175
TATTATC553.218791E-452.721582
CTTCCGA13600.052.2428471
TCCGATC13500.052.0869673
TTCCGAT13700.051.326582
CCGATCT13850.051.29414
TAGACTG450.0096397948.3281145
TGTAGGG1002.732766E-643.4953042
GATCTGA2300.040.973846
TACAGGA1303.695386E-739.034252
ATCTAGG750.001483295338.6624952
ATCTGAG951.0286334E-438.1537747
GATCTAG1209.551786E-636.2501371
CGATCTA3100.035.076865
CGATCTG9950.033.513975
GATCTAC2451.9681465E-929.5886426
TACGTAG1753.7683676E-629.0001131
ATCTACA1255.152596E-428.996877
TATAGGT1504.3965752E-528.996873
TGTAATA1000.0060741228.996872
Produced by FastQC (version 0.11.2)