FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4a7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4a7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences609091
Sequences flagged as poor quality0
Sequence length151
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG28310.46479097540433206No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG25030.41094023717309897No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC24020.3943581500957985No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC15470.25398503671865125No Hit
GGTAGGACGTGTCGGCTGCTTTGTTGAGCCGTCACACGGAATCGAGAGCT14300.23477608436177846No Hit
CTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTCTTAATCG12850.21097011776565408No Hit
ATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAGCTCG12780.20982086420584115No Hit
GTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCT12600.2068656407663223No Hit
CTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTT12350.20276116376699047No Hit
GATTAGAGGCATTGGGGGCGCAACGCCCTCGACCTATTCTCAAACTTTAA12170.19980594032747162No Hit
GTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGCGG11930.1958656424081131No Hit
TCTCCGGACTCCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAA11840.1943880306883536No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAAT11630.19094027000891495No Hit
GTCTTAATCGACCAACACCCTTTGTGGGTTCTAGGTTAGCGCGCAGTTGG11280.1851940022098504No Hit
GTGATAAAGGAGGTAGGGGTGCAGAGACAGCCAGGAGGTTTGCCTAGAAG11230.18437310680998406No Hit
GTGCCCAACTGCGCGCTAACCTAGAACCCACAAAGGGTGTTGGTCGATTA11020.18092534613054534No Hit
TTGTTACACACTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCT10470.1718954967320154No Hit
GTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGC10420.17107460133214905No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGC10340.16976116869236288No Hit
CTCCTACTCATCGAGGCCTGGCTCTTGCCCCGACGGCCGGGTATAGGTCG10060.16516415445311128No Hit
GTACAGGAATATTCACCTGTTGTCCATCGACTACGCCTTTCGGCCTGATC9930.16302982641345876No Hit
CCCATGTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCGGCCTTCA9740.1599104238939666No Hit
GTTACACACTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGT9660.15859699125418042No Hit
AGGTAGGACGTGTCGGCTGCTTTGTTGAGCCGTCACACGGAATCGAGAGC9650.15843281217420713No Hit
TTTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGATAACTCTTCCCGAGGC9420.15465669333482188No Hit
GATCTGCACCGACGGCCGCTCCGCCCGGGCTCGCGCCCTAGGTTTTGCAG9170.1505522163354901No Hit
CTATCAGACGGGTTTCCCCCGACTCTTAGGATCGACTAACCCATGTGCAA8610.1413581878569869No Hit
CCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTCTTAATCGAC8530.14004475521720072No Hit
GGGTAAAGCCAGAGGAAACTCTGGTGGAAGCCCGCAGCGATACTGACGTG8210.134791024658056No Hit
TCCATGACCACCGTCCTGCTGTCTTAATCGACCAACACCCTTTGTGGGTT7990.13117908489864405No Hit
GGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCC7990.13117908489864405No Hit
CCCTTGAAAGAGTGCGTAATAGCTCACTGATCGAGCGCTCTTGCGCCGAA7830.1285522196190717No Hit
GTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCG7810.12822386145912515No Hit
GATTAAGACAGCAGGACGGTGGTCATGGAAGTCGAAATCCGCTAAGGAGT7440.12214923550011411No Hit
AACCTAGAACCCACAAAGGGTGTTGGTCGATTAAGACAGCAGGACGGTGG7260.11919401206059521No Hit
CTCTTAGGATCGACTAACCCATGTGCAAGTGCCGTTCACATGGAACCTTT7050.1157462513811565No Hit
GTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCGGCCTTCAAAGTT6720.11032834174203854No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC6550.10753729738249293No Hit
ATGAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGG6530.1072089392225464No Hit
ATCTTAGGCCCTGACTCACCCTCCGTGGACGAACCTTGCGGAGGAACCCT6530.1072089392225464No Hit
ATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGGCGCG6450.10589550658276022No Hit
CCCTTTGTGGGTTCTAGGTTAGCGCGCAGTTGGGCACCGTAACCCGGCTT6450.10589550658276022No Hit
CTTAAGCGCTATCAGACGGGTTTCCCCCGACTCTTAGGATCGACTAACCC6430.10556714842281367No Hit
TTCTAGGTTAGCGCGCAGTTGGGCACCGTAACCCGGCTTCCGGTTCATCC6410.10523879026286713No Hit
ACCTAGGGCGCGAGCCCGGGCGGAGCGGCCGTCGGTGCAGATCTTGGTGG6350.1042537157830275No Hit
CCTAGAACCCACAAAGGGTGTTGGTCGATTAAGACAGCAGGACGGTGGTC6350.1042537157830275No Hit
CCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAA6330.10392535762308096No Hit
GAATCGAGAGCTCCAAGTGGGCCATTTTTGGTAAGCAGAACTGGCGATGC6320.10376117854310768No Hit
ACCAAGATCTGCACCGACGGCCGCTCCGCCCGGGCTCGCGCCCTAGGTTT6280.10310446222321458No Hit
GCGCTTAAGCGCGCGACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCT6250.10261192498329477No Hit
ATTTTAACCGGATCCCCTTTCGAAGTTCGCGCTTAAGCGCTATCAGACGG6160.10113431326353532No Hit
ACCTAGAACCCACAAAGGGTGTTGGTCGATTAAGACAGCAGGACGGTGGT6120.10047759694364225No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA19100.058.4635431
TTCCGAT19100.058.079142
TAGACGT1101.6734703E-952.73024
CCGATCT22250.050.5084154
TCCGATC23500.047.513283
ATCTAAC700.001056880741.424077
TGCTCTT11450.040.5262682
GTGCTCT11700.040.283321
CGATCTA3700.039.191365
GTATACT800.00203543636.252014
TGTAAGG800.00203543636.252012
TCTTAGC1001.3917006E-436.2460568
TCTTCCG13000.035.1365625
AATACTA1251.2637962E-534.801935
GCTATGT1601.8735082E-631.7231161
ATCTGTG1652.3864286E-630.7542327
GCTCTTC15450.030.5033133
GTATATA3350.030.3026771
AGGGGTA3850.030.1315424
CATACCC1453.486855E-530.0016654
Produced by FastQC (version 0.11.2)