FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4a6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4a6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1049020
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC22190.21153076204457494No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA18340.1748298411851061No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG16830.16043545404282092No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA16680.15900554803530914No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC15910.15166536386341536No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT15590.14861489771405692No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT14510.13831957445997214No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC12550.11963546929515166No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT12400.11820556328763988No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT11730.11181864978742065No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT11600.11057939791424377No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT10700.10199996186917312No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA13200.052.182441
CCGATCT13150.051.2780954
TTCCGAT13400.050.8625072
CGATCTG8250.040.4276585
TCGCGCG6300.037.972169
TCCCCCC8400.037.9721578
GTCGCGC7250.033.9963878
CCCCCCG9450.033.753039
TATAGGT7500.032.8694463
ACTCCCC10000.031.9011756
GGTCGCG7750.031.8030747
AGGTCGC7900.031.2036826
ACCTATA7950.031.0089111
CTCCCCC10250.030.4114047
GTATAGA4350.030.0025351
GGGCGAC4400.029.6559417
ATACTCC11150.028.6122824
ATACCCG8450.028.3160025
CCTACAT2903.947207E-1027.5023233
CCATACT11350.027.469282
Produced by FastQC (version 0.11.2)