FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4a5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4a5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1390106
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC20100.14459329000810012No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA19810.1425071181622121No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG17350.1248106259522655No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT16050.11545882112587097No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA15700.11294102751876475No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC15200.10934417950861301No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT14470.10409278141379147No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCCCC9950.038.615058
CTTCCGA16750.038.5318341
CCGATCT17350.036.3620724
CCCCCCG10550.035.731799
TTCCGAT18450.034.5859342
CTCCCCC11450.032.9231767
GTCGCGC6850.029.6327068
TCGCGCG7100.029.6103489
CGATCTG10750.029.0060675
ACTCCCC14200.027.0664946
ACCATAC14500.027.006621
ATACTCC14600.026.8206774
GGTCGCG7600.026.7084267
CATACTC16550.026.2894863
TATAGGT8600.026.1391873
TACTCCC16100.026.1234745
AGGTCGC8150.025.8038716
GTACAGG3709.094947E-1225.479221
CCATACT15100.025.4514272
CTATAGG2301.101278E-625.2235741
Produced by FastQC (version 0.11.2)