FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4a4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4a4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences271589
Sequences flagged as poor quality0
Sequence length151
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG7650.28167562014661857No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGC5070.18667913648932763No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG4870.17931506798876243No Hit
TCTCCGGACTCCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAA4160.15317262481175598No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC3900.14359933576102124No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGC3660.13476245356034303No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC3490.1285029953348626No Hit
GTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGCGG3420.12592557135966478No Hit
TTTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGATAACTCTTCCCGAGGC3350.12334814738446696No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA3060.11267024805864745No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2910.10714719668322355No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT2840.10456977270802573No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCTA450.00960671648.365035
GGTAACC657.330042E-444.611767
CTTCCGA13000.040.738231
TTCCGAT13500.039.229412
CCGATCT13700.038.1271744
GATCTTT3800.036.273776
TCCGATC14900.035.5434273
ATAGTCC1051.8510553E-434.5210048
TAGAGCA850.00272969534.1400224
CTTATAA1102.4300323E-432.957935145
CGATCTT6500.032.3673635
GTACACC900.00361199632.243354
CCTATCA1801.2791315E-732.243352
ACAGGGG1153.1439657E-431.5424083
CGATCTA2802.582965E-1028.500825
TCAGTAC1050.007674132527.6371573
TCTGTCC1050.007701783427.6168028
TATAGGT3700.027.450423
TCCTATC1606.7797955E-527.2053281
ACGAGTC2156.062164E-726.9944345
Produced by FastQC (version 0.11.2)