FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4a2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4a2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences929819
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC18380.19767288042081307No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA17140.1843369516002577No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG16050.17261423997573722No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT15270.1642255105563556No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA14500.15594432895004298No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC13080.14067253949424566No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT13050.14034989605503867No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC11180.12023845501113657No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT10900.11722711624520471No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT10880.11701202061906671No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGT10090.10851574338661611No Hit
CATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTT10010.10765536088206415No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCCCC8150.048.0338758
CTTCCGA10550.042.6154861
CCCCCCG9550.040.9922569
TTCCGAT11900.037.7809562
CCGATCT11800.036.8720664
ATACTCC11400.036.257534
CTCCCCC11250.034.1534657
CCATACT11300.033.3697622
CATACTC12000.033.236073
ACTCCCC11800.033.184866
ACCATAC11250.032.8734931
TCGCGCG6100.032.08829
GTCGCGC6000.031.4147438
CGATCTG7150.030.4259015
TACTCCC13750.030.0607915
GGTCGCG6300.029.9188027
CCTACAC7350.029.5979823
TACACCT7700.028.252625
TCTAGGG2603.7107384E-927.890412
TATAGGT7050.027.7717273
Produced by FastQC (version 0.11.2)