FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4a1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4a1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences700367
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG9450.1349292585173202No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA8300.11850929584060928No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC8070.1152253033052671No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA7060.10080429260659055No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCCG5300.031.4578659
TCCCCCC5300.030.090138
ACCATAC6300.027.6211011
ACTACGC1050.007712161627.6151858
ATACTCC6550.026.5649624
CTCCCCC6050.026.3599517
CCATACT6450.024.7287542
ACTCCCC6600.024.1667396
GTGTTAC2852.7743408E-722.896441
CATACTC7800.022.307763
AGAGCGC1952.6161328E-422.3045738
TAGGACA3655.677066E-921.8493794
TTAGGAC4006.89397E-1021.7500673
ATAATAC4801.4551915E-1121.1458973
ATATAAC3106.238279E-721.0484493
CAGTCCT2104.3164258E-420.711399
TATGCAC2505.9710877E-520.300063
TACTCCC9000.020.138955
ATCCTAC5505.456968E-1219.7741971
CCTAATA2205.9008214E-419.7727872
Produced by FastQC (version 0.11.2)