Basic Statistics
Measure | Value |
---|---|
Filename | H3VL5AFXY_n01_p4a12.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 134573 |
Sequences flagged as poor quality | 0 |
Sequence length | 151 |
%GC | 48 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC | 250 | 0.18577277760026156 | No Hit |
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA | 230 | 0.17091095539224066 | No Hit |
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA | 199 | 0.1478751309698082 | No Hit |
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT | 195 | 0.14490276652820402 | No Hit |
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT | 193 | 0.14341658430740192 | No Hit |
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC | 179 | 0.1330133087617873 | No Hit |
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT | 169 | 0.12558239765777682 | No Hit |
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG | 161 | 0.11963766877456845 | No Hit |
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC | 155 | 0.11517912211216218 | No Hit |
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT | 151 | 0.11220675767055797 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TAGCTCG | 25 | 9.35924E-4 | 87.02275 | 4 |
TTCTAAA | 90 | 2.0274456E-8 | 56.403637 | 2 |
GACTGTA | 40 | 0.0060421894 | 54.36901 | 7 |
ACTGTAA | 55 | 3.2107293E-4 | 52.721462 | 8 |
ACACCGT | 70 | 1.6854712E-5 | 51.799255 | 6 |
GTCTACG | 115 | 2.4501787E-9 | 50.44797 | 1 |
ATATAGG | 45 | 0.009611214 | 48.345974 | 6 |
ATAAGAC | 45 | 0.009611214 | 48.345974 | 3 |
GACTGGC | 45 | 0.009625345 | 48.328007 | 7 |
TATATAG | 65 | 7.3044567E-4 | 44.627052 | 5 |
ATTGAAC | 65 | 7.3044567E-4 | 44.627052 | 3 |
CCTACAC | 85 | 5.308581E-5 | 42.658215 | 3 |
CGAGTCG | 140 | 1.3957106E-8 | 41.439407 | 6 |
AGGACTG | 70 | 0.0010520255 | 41.439407 | 5 |
ATACTCC | 90 | 7.436432E-5 | 40.28831 | 4 |
TCCCCCC | 90 | 7.452756E-5 | 40.27334 | 8 |
TACGAGT | 145 | 1.9022991E-8 | 40.01046 | 4 |
TCTAAAG | 130 | 3.6591337E-7 | 39.04867 | 3 |
CTACACC | 115 | 7.084036E-6 | 37.83598 | 4 |
ACGAGTC | 155 | 3.4242476E-8 | 37.42914 | 5 |