FastQCFastQC Report
Fri 10 Aug 2018
H3VL5AFXY_n01_p4a12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VL5AFXY_n01_p4a12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134573
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC2500.18577277760026156No Hit
CCTATAAGCAACATCCGCTGATCCCTGGTCGGCATCGTTTATGGTTGAGA2300.17091095539224066No Hit
GGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA1990.1478751309698082No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT1950.14490276652820402No Hit
GTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACT1930.14341658430740192No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC1790.1330133087617873No Hit
GACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGAT1690.12558239765777682No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAG1610.11963766877456845No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTC1550.11517912211216218No Hit
TCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGAT1510.11220675767055797No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCTCG259.35924E-487.022754
TTCTAAA902.0274456E-856.4036372
GACTGTA400.006042189454.369017
ACTGTAA553.2107293E-452.7214628
ACACCGT701.6854712E-551.7992556
GTCTACG1152.4501787E-950.447971
ATATAGG450.00961121448.3459746
ATAAGAC450.00961121448.3459743
GACTGGC450.00962534548.3280077
TATATAG657.3044567E-444.6270525
ATTGAAC657.3044567E-444.6270523
CCTACAC855.308581E-542.6582153
CGAGTCG1401.3957106E-841.4394076
AGGACTG700.001052025541.4394075
ATACTCC907.436432E-540.288314
TCCCCCC907.452756E-540.273348
TACGAGT1451.9022991E-840.010464
TCTAAAG1303.6591337E-739.048673
CTACACC1157.084036E-637.835984
ACGAGTC1553.4242476E-837.429145