FastQCFastQC Report
Fri 28 Sep 2018
H3VH3AFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VH3AFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14524327
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1621891.1166713610895707No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG64750.07.024868570
GTAGGGG77100.06.76186670
GCCTGTG45900.06.70817970
CGCCTGT36100.06.688644469
TCGCCTG44400.06.541711369
TCGTGTT193150.06.322158370
GGGAAAA124900.06.135025570
CCTGTGT45750.05.96538170
GTCTTCG73800.05.54707670
AGAGCGG96100.05.534270
TTCGCCG43250.05.420300570
CTTCGCC67700.05.323328570
GTGTAGA101600.05.16573670
GAGTGTG116150.05.151237570
AGAGTGT339900.05.14699270
Produced by FastQC (version 0.11.5)