FastQCFastQC Report
Fri 28 Sep 2018
H3VH3AFXY_n02_pIXHALO_col_wg-a-cut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VH3AFXY_n02_pIXHALO_col_wg-a-cut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3538336
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG65980.18647183308764345No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG9700.015.87616370
TGTACGG17200.012.20920770
GTACGGG5451.0913936E-1112.20173970
ACGTCCT23900.011.27606269
ACGTGCG5451.6989361E-910.91734670
TACGGGC4005.179021E-610.4996266
CGTCCGG7807.2759576E-129.87171770
CCTGGTG19900.09.49741369
GTACGTG15350.09.3484670
TACGTCC34600.09.30628568
GTCCGGG7706.1118044E-109.09083869
CTGGTGT22650.08.96240270
GTAGGGG21650.08.89138570
ACGGGCT4351.4804321E-48.85050567
CCGGGTG4855.066098E-58.65972569
CGTCCTG33600.08.541670
GCGTCGG14050.08.22057570
GAGCGTG8951.1841621E-98.21222670
GTACGTC39250.08.11458667
TACGGCC7603.7856626E-77.82888670
Produced by FastQC (version 0.11.5)