FastQCFastQC Report
Fri 28 Sep 2018
H3VH3AFXY_n02_pIXHALO_col_rr-a-cut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VH3AFXY_n02_pIXHALO_col_rr-a-cut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4016353
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG77100.19196519827813938No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGGG4601.8049832E-811.41270170
GTACGTG25300.011.20519670
GTAGGGG16300.010.52115670
CAGTACG31400.010.47739270
TACGTGT23450.09.999770
AGTACGT30150.09.75095169
TGTCAGT61300.09.53478670
TCAGTAC42500.09.05855270
GTCAGTA52600.08.98261969
GTGTCAG72200.08.77397469
AGTACGG8001.2569217E-98.74973770
ACGTGTT12100.08.67742570
TCGTGTT67500.08.50344870
GCGTCGG10202.2737368E-107.8919270
AGAGCGG16100.07.60846770
TGTGTCA86400.07.4534868
GTGTGTC81550.07.253000767
GGGAAAA42000.07.16645170
GTAGGGT22650.06.79891370
AGTGTGT122100.06.707415670
Produced by FastQC (version 0.11.5)