FastQCFastQC Report
Fri 28 Sep 2018
H3VH3AFXY_n02_ZmTCP11_retic_NYU6-cut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VH3AFXY_n02_ZmTCP11_retic_NYU6-cut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12984453
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG315250.24279035859269543No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGTG133150.011.38135970
TCGCCTG96600.010.90527970
TTCGCCG67900.010.72114170
CGCCTGT77850.09.93530270
GCCTGTG87000.09.81562570
CCTGTGT75000.09.61287770
CTTCGCC159850.08.86726770
TTCGCCT110600.08.82893269
TCGTGTT216150.08.58159570
CTGTGTA72100.08.25203570
TCTTCGC177350.07.95282270
GCGTCGG39400.07.905715570
GGGAAAA130750.07.89637470
GTCTTCG201700.07.8603170
TAGCCTG37550.07.642779470
AGAGCGG56500.07.061610770
TGTCTTA100100.06.922747670
GTGTAGA89600.06.796551770
GAGTGTC364000.06.70178569
AGTGTCG63600.06.54843170
Produced by FastQC (version 0.11.5)