FastQCFastQC Report
Fri 28 Sep 2018
H3VH3AFXY_n02_ZmTB1_retic_NYU4-cut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VH3AFXY_n02_ZmTB1_retic_NYU4-cut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15592441
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG363740.23327970264566017No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCCG76350.012.56022970
TCGCCTG122850.011.31022870
CGCCTGT95000.010.79443770
CCTGTGT103950.010.67310570
GAGTGTG161350.010.60708270
GCCTGTG114200.09.53132269
CTTCGCC203200.08.99089370
CTGTGTA91100.08.95145370
GTAGGGG69350.08.93270170
TTCGCCT141400.08.61367869
TCTTCGC235400.08.4895770
CGCCAGT33450.08.2658470
GGGAAAA167900.08.00454370
TCGTGTT271200.07.93672770
TAGCCTG49500.07.918973470
GAGTGTA110050.07.823570
GTCTTCG267650.07.806620670
GCGTCGG39300.07.658821670
TCGCCAG51600.07.528861570
TGTCTTA133100.07.126023370
Produced by FastQC (version 0.11.5)