FastQCFastQC Report
Fri 28 Sep 2018
H3VH3AFXY_n02_ZmLG1_retic_NYU3-cut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VH3AFXY_n02_ZmLG1_retic_NYU3-cut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12216523
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG430370.3522851796701893No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGTG91750.09.84178970
TCGCCTG72300.09.29444370
CGCCTGT62600.09.05734970
TCGTGTT139900.07.980560370
GCCTGTG69650.07.437055670
TTCGCCG63650.07.423279370
GTAGGGG50550.07.408376770
CCTGTGT64750.07.40527670
TTCGCCT84050.07.32887269
TCTTCGC128000.07.163937670
CTTCGCC125200.06.820966770
GTCTTCG141850.06.661849570
AGTGTCT218350.06.620016669
GAGTGTC240200.06.411237768
TGTCTTA68100.06.321475570
GTGTCTT239000.06.194452370
GTGTAGA65550.06.03345770
AGAGTGT351600.06.03234269
GGGAAAA92000.05.70642270
CTGTGTA62750.05.633367570
Produced by FastQC (version 0.11.5)