FastQCFastQC Report
Fri 28 Sep 2018
H3VH3AFXY_n02_ZmKN1_retic_NYU1-cut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VH3AFXY_n02_ZmKN1_retic_NYU1-cut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9564964
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG331910.3470060106865013No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCCG47900.013.88298670
TCGCCTG82150.012.18493770
GAGTGTG107150.011.79179270
GCCTGTG74200.010.66029770
CGCCTGT61700.010.60775669
CCTGTGT65550.010.57200370
TTCGCCT92650.010.04854869
GTAGGGG50600.09.47621370
GCCGGTG29700.09.19185270
CTTCGCC127300.09.18296370
TCTTCGC147850.08.71146770
GGGAAAA99900.08.68862370
CTGTGTA59750.08.08362270
TCGTGTT187100.08.00635370
GTCTTCG168300.07.652983769
GCCAGTG28300.07.420439770
AGAGCGG46850.07.32118370
CGCCGGT26950.07.27271169
GAGTGTA67000.07.208901470
AGTGTCT289600.07.082166769
Produced by FastQC (version 0.11.5)