FastQCFastQC Report
Fri 28 Sep 2018
H3VH3AFXY_n02_ZmHB54_retic_NYU7-cut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VH3AFXY_n02_ZmHB54_retic_NYU7-cut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11823038
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG292960.24778741301516582No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG40000.09.88728170
GTAGGGG60350.08.98903170
AGAGCGG64050.08.57904570
TCGTGTT194100.07.44702770
GAGTGTT288900.06.90535170
GTTAAGA201150.06.803230369
GAGCGTG25350.06.765136770
TAAGATT190050.06.70335170
AGATTAG126700.06.602063769
GTGTTAA225850.06.601580670
GATTAGT126500.06.446497470
TGTTAAG199700.06.414479768
AGTGTTA238650.06.26217169
AGAGTGT317750.06.1792569
AAGAGTG388800.05.797197370
AGTGTAG65100.05.75256169
CGGAAGC94550.05.700561570
TTAAGAT242150.05.57906370
GGAGGGG72850.05.572972370
GTGTAGA69150.05.21318970
Produced by FastQC (version 0.11.5)