FastQCFastQC Report
Fri 28 Sep 2018
H3VH3AFXY_n02_ZmHB117_FBR1_retic_NYU5-cut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VH3AFXY_n02_ZmHB117_FBR1_retic_NYU5-cut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16152171
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG395270.24471632946431784No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTG98200.011.1909370
CCTGTGT84750.010.57178470
GAGTGTG136650.010.34712770
TTCGCCG66650.09.92452470
GTAGGGG60650.09.80993670
GCCTGTG92900.09.60675169
CGCCTGT75050.09.51321970
TTCGCCT122900.08.34387569
CTTCGCC166100.08.07006870
TCGTGTT219750.07.899522370
GCGTCGG37200.07.71469870
TCTTCGC186500.07.656483770
AGAGCGG58000.07.482413370
GGGAAAA139150.07.26879970
GCCGGTG41600.07.15111370
GTCTTCG207750.07.092398669
CGCCGGT36000.06.902459670
CTGTGTA78650.06.808332470
GAGTGTA90500.06.80631670
GAGTGTC358500.06.55066669
Produced by FastQC (version 0.11.5)