FastQCFastQC Report
Fri 28 Sep 2018
H3VH3AFXY_n02_ZmBZIP2_DFL1_retic_NYU8-cut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VH3AFXY_n02_ZmBZIP2_DFL1_retic_NYU8-cut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16302460
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG355010.21776468091318732No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGGG98200.09.87247870
GATTAGT182350.08.86735470
GCGTCGG72850.08.31140570
ATTAGTG166350.08.289556570
AGAGCGG104300.08.12061570
AGATTAG216850.07.81164569
TAAGATT314300.07.51652870
TCGTGTT325350.07.497903370
AGTGTAG103950.07.306226370
GTGTTAA420050.07.282295770
GAGCGTG40900.06.931378470
GTTAAGA366700.06.871941670
AGATTAT131000.06.839534870
TAGTGTA135950.06.79643769
TGTTAAG368900.06.73606569
AGTGTTA448350.06.674296469
AAGATTA277400.06.409347569
GAGTGTT549400.06.389548370
AGAGTGT605350.06.336681470
GTGTAGA108800.05.918978770
Produced by FastQC (version 0.11.5)