FastQCFastQC Report
Fri 28 Sep 2018
H3VH3AFXY_n02_ZmBBM_retic_NYU2-cut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VH3AFXY_n02_ZmBBM_retic_NYU2-cut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8939969
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG383110.42853616159071695No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGTGT108900.06.20279369
GAGTGTG49450.06.01610470
AAAGAGT135950.05.50931170
AGTGTCT78350.05.449776669
CCTGTGT31600.05.42714970
TCGTGTT63300.05.418575370
AAGAGTG123650.05.349652368
GAGTGTC84600.05.088533468
GCGTGCC77550.05.01053521
Produced by FastQC (version 0.11.5)