FastQCFastQC Report
Fri 28 Sep 2018
H3VH3AFXY_n02_AtTGA5_col_wg-a-cut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VH3AFXY_n02_AtTGA5_col_wg-a-cut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2423603
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50710.20923393806658927No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG5150.023.10668670
TGTACGG11200.012.49994170
TACGTCC35250.011.12051570
CTGGTGT17650.010.90646469
GTCCGGG3702.5159668E-510.40535668
CCTGGTG15800.010.18982570
CCGGGTG3106.009028E-410.16124370
GTACGGG3106.009028E-410.16124369
GTACGTC39950.010.07504670
ACGTCCT23500.09.97867769
GCGTCGG5401.893186E-79.72217770
GGGAAAA25450.09.62667470
CGTCCAG6603.8544385E-99.54540969
TGTACGT55050.09.346004569
GTAGGGG8657.2759576E-129.30631570
TCCTGGG4158.8647954E-59.27706570
CGTCCGG3854.2845577E-49.09086667
GTCCTGG25650.08.86935370
GTGTAGA18500.08.702661570
GTCCAGG6959.0114554E-88.561110570
Produced by FastQC (version 0.11.5)