FastQCFastQC Report
Fri 28 Sep 2018
H3VH3AFXY_n02_AtTGA5_col_rr-a-cut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VH3AFXY_n02_AtTGA5_col_rr-a-cut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3542744
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG71190.2009459334346484No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACGG8250.015.27248170
GTACGTG18950.011.08161570
TCAGTAC32900.010.95727170
CAGTACG23100.010.908915569
GTCAGTA39750.010.83001369
GTAGGGG20350.010.49123170
GCGTCGG13450.010.40875470
AGTACGT23250.010.386929570
TACGTGT18100.09.66835270
AGAGCGG21900.09.10944370
TGTCAGT48200.08.64093968
TGTCAGG12000.08.16653470
GAGTACG12050.08.13264869
GTGTCAG58900.08.08136469
GTGTGTC68300.07.430334669
ACGTGTA21050.07.315796470
GTACGGG4800.0037891957.291548767
CGGACGA5300.00123621017.263828852
TGTGTCA70300.07.11937370
GTGTGTG38150.07.06410670
Produced by FastQC (version 0.11.5)