FastQCFastQC Report
Fri 28 Sep 2018
H3VH3AFXY_n02_AtSPL9_col_wg-a-cut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VH3AFXY_n02_AtSPL9_col_wg-a-cut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3663935
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG67070.18305455746349214No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG10100.018.7123870
CGGGTGT6900.011.6663670
CCGGGTG5451.6971171E-910.91744269
GTAGGGG20850.010.74312270
ACGTCCT27800.010.32375169
CTGGTGT23100.09.69671470
TACGTCC39950.09.46183669
GAGCGTG7604.765752E-109.21028470
CGTCCTG33100.08.98767970
AGAGCGG19600.08.92833670
ACGTGCG4804.4873857E-58.7497770
ACGGGCG4855.0673465E-58.65956670
TGTACGG27900.08.53024270
TCCTGGG7909.282303E-98.417570
TCGTGTT69450.08.41590570
GTCCGGG7101.272656E-78.38006170
CCTGGTG19900.08.26633969
GTACGTC46300.08.16415568
TACGGCC7051.0213407E-67.943269768
GTACGTG19100.07.696132770
Produced by FastQC (version 0.11.5)