FastQCFastQC Report
Fri 28 Sep 2018
H3VH3AFXY_n02_AtSPL9_col_rr-a-cut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VH3AFXY_n02_AtSPL9_col_rr-a-cut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3154150
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50320.15953584959497805No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTGT18150.011.37718970
GTAGGGG12750.010.15666570
GTACGTG20300.010.00012369
GGGAAAA26500.09.90546770
TCGTGTT49350.09.64520370
GTCAGTA32900.08.72323570
CAGTACG23550.08.62006369
TGTCAGT36550.08.42691669
AGAGCGG12850.08.17104770
AGTACGT25650.08.05052670
GTGTCAG43600.07.545964269
AGAGTGT101250.07.431953470
GCGTCGG8502.8272189E-77.411620670
GTGTAGA18450.07.398229670
GTGTGTG29800.07.04684270
AAGAGTG114750.06.801826569
TCAGTAC33600.06.77091669
ACGTGTA18650.06.75632968
GTGTGTC51350.06.748105568
AGTGTGG13351.3587851E-96.554179770
Produced by FastQC (version 0.11.5)