FastQCFastQC Report
Fri 28 Sep 2018
H3VH3AFXY_n02_AtSPL11_col_wg-a-cut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VH3AFXY_n02_AtSPL11_col_wg-a-cut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3917811
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG83420.2129250236930776No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG14200.09.858767570
GTAGGGG20050.08.90239270
GATTAGT48350.08.75870370
TCGTGTT76600.07.99576970
AGATTAG55300.07.911080469
AGTGTAG26300.07.71832769
GTGTTAA117850.07.69167969
TAAGATT84700.07.68564870
GTGTAGA29450.07.36813170
ATTAGTG43550.07.2327870
GTTAAGA97750.07.08923570
AGTGTTA128000.07.0544168
AAGATTA72700.07.02860969
GTGCCGT5050.00620658146.93077418
AGAGCGG21850.06.727423770
TGTTAAG102250.06.606096769
GGGAAAA36400.06.538204770
AAGGCCT8106.047539E-56.48180534
AGATTAT34100.06.466020670
GAGTGTT148400.06.462009470
Produced by FastQC (version 0.11.5)